Expression of the kanamycin resistance gene in a kanamycin-producing strain of Streptomyces kanamyceticus.
نویسندگان
چکیده
منابع مشابه
Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species.
A kanamycin-producing strain, Streptomyces kanamyceticus ISP5500, is resistant to kanamycin. A kanamycin resistance determinant was cloned from S. kanamyceticus into Streptomyces lividans 1326, using the plasmid vector pIJ702. The resulting plasmid, pMCP5, could also transform Streptomyces lavendulae S985 and Streptomyces parvulus 2283 to kanamycin resistance. Transformants carrying pMCP5 were ...
متن کاملThe kanamycin biosynthetic gene cluster from Streptomyces kanamyceticus.
Kanamycin is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus1). Although it possesses broad range antibacterial activity, bacteria resistant to this antibiotic readily appear, and therefore its clinical use has been restricted to tuberculosis. Resistant bacteria inactivate kanamycin by modifying its specific aminoor hydroxygroup using acetyltransferase, phosphotransferase an...
متن کاملAmplification of the entire kanamycin biosynthetic gene cluster during empirical strain improvement of Streptomyces kanamyceticus.
Streptomyces kanamyceticus 12-6 is a derivative of the wild-type strain developed for industrial kanamycin (Km) production. Southern analysis and DNA sequencing revealed amplification of a large genomic segment including the entire Km biosynthetic gene cluster in the chromosome of strain 12-6. At 145 kb, the amplifiable unit of DNA (AUD) is the largest AUD reported in Streptomyces. Striking rep...
متن کاملModulation of kanamycin B and kanamycin A biosynthesis in Streptomyces kanamyceticus via metabolic engineering
Both kanamycin A and kanamycin B, antibiotic components produced by Streptomyces kanamyceticus, have medical value. Two different pathways for kanamycin biosynthesis have been reported by two research groups. In this study, to obtain an optimal kanamycin A-producing strain and a kanamycin B-high-yield strain, we first examined the native kanamycin biosynthetic pathway in vivo. Based on the prop...
متن کاملMineral nutrition of Streptomyces kanamyceticus for kanamycin formation.
Kanamycin production by Streptomyces kanamyceticus ATCC 12853 requires magnesium sulfate and potassium phosphate at concentrations of 0.4 and 1.0 g per liter, respectively. The optimal concentrations of Fe and Zn for production of kanamycin are 0.25 and 0.575 mug/ml, respectively, whereas Mo at 0.04 mug/ml allows maximal cellular growth and antibiotic synthesis. Mn and Ca are without any effect...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1989
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.42.423